LOCUS       40924_11471       10437 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gene trap vector pCMT-SAhygpA-NP21 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10437)
  AUTHORS   Horie K, Kokubu C, Yoshida J, Akagi K, Isotani A, Oshitani A, Yusa 
            K, Ikeda R, Huang Y, Bradley A, Takeda J.
  TITLE     A homozygous mutant embryonic stem cell bank applicable for 
            phenotype-driven genetic screening
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10437)
  AUTHORS   Horie K, Takeda J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2011) Contact:Kyoji Horie Osaka University, 
            Department of Social and Environmental Medicine, Graduate School of 
            Medicine; 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
REFERENCE   3  (bases 1 to 10437)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10437)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2011) Contact:Kyoji Horie Osaka University, Department of 
            Social and Environmental Medicine, Graduate School of Medicine; 2-2 
            Yamadaoka, Suita, Osaka 565-0871, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        745..1124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1125..1328
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    1342..1409
                     /label=R region of Moloney Murine Leukemia Virus
                     /note="R region of Moloney Murine Leukemia Virus"
     misc_feature    1410..1484
                     /label=U5 region of Moloney Murine Leukemia Virus
                     /note="U5 region of Moloney Murine Leukemia Virus"
     misc_feature    1549..1906
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1965..2381
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    2391..2765
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     polyA_signal    complement(2801..3025)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3036..3069)
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             3086..3679
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             3686..4678
                     /codon_start=1
                     /label=Delta-TK
                     /note="truncated herpes simplex virus thymidine kinase
                     (Salomon et al., 1995)"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             complement(4685..5485)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     protein_bind    5502..5535
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        complement(5540..6038)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    complement(6065..6275)
                     /label=contains phosphoglycerate kinase 1 polyA signal
                     /note="contains phosphoglycerate kinase 1 polyA signal"
     CDS             complement(6282..7304)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    complement(7337..7440)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     protein_bind    complement(7817..7850)
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    8050..8117
                     /label=R region of Moloney Murine Leukemia Virus
                     /note="R region of Moloney Murine Leukemia Virus"
     misc_feature    8118..8190
                     /label=U5 region of Moloney Murine Leukemia Virus
                     /note="U5 region of Moloney Murine Leukemia Virus"
     promoter        complement(8249..8267)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8288..8304)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8312..8328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8336..8366)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8381..8402)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8690..9278)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9452..10309)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10310..10414)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggcctg gccattgcat acgttgtatc catatcataa tatgtacatt tatattggct
      721 catgtccaac attaccgcca tgttgacatt gattattgac tagttattaa tagtaatcaa
      781 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      841 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      901 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      961 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     1021 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     1081 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     1141 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     1201 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1261 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1321 gcagagctcg tttagtgaac cgcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     1381 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     1441 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg
     1501 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc
     1561 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg
     1621 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg
     1681 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc
     1741 gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc taataggagg
     1801 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt
     1861 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt
     1921 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca
     1981 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt
     2041 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta
     2101 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca
     2161 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg
     2221 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc
     2281 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc
     2341 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga
     2401 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta
     2461 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct
     2521 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt
     2581 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct
     2641 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg
     2701 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta
     2761 gactgccgga tctagctagt tatcgagtcc gcctcagaag ccatagagcc caccgcatcc
     2821 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     2881 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     2941 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cgggggaggg gcaaacaaca
     3001 gatggctggc aactagaagg cacagtcgag gctagataac ttcgtatagg atactttata
     3061 cgaagttatt ggatccacca tggggaccga gtacaagccc acggtgcgcc tcgccacccg
     3121 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3181 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     3241 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     3301 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3361 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3421 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3481 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3541 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     3601 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     3661 gacccgcaag cccggtgccg gctccatgcc cacgctactg cgggtttata tagacggtcc
     3721 ccacgggatg gggaaaacca ccaccacgca actgctggtg gccctgggtt cgcgcgacga
     3781 tatcgtctac gtacccgagc cgatgactta ctggcgggtg ctgggggctt ccgagacaat
     3841 cgcgaacatc tacaccacac aacaccgcct cgaccagggt gagatatcgg ccggggacgc
     3901 ggcggtggta atgacaagcg cccagataac aatgggcatg ccttatgccg tgaccgacgc
     3961 cgttctggct cctcatatcg ggggggaggc tgggagctca catgccccgc ccccggccct
     4021 caccctcatc ttcgaccgcc atcccatcgc cgccctcctg tgctacccgg ccgcgcggta
     4081 ccttatgggc agcatgaccc cccaggccgt gctggcgttc gtggccctca tcccgccgac
     4141 cttgcccggc accaacatcg tgcttggggc ccttccggag gacagacaca tcgaccgcct
     4201 ggccaaacgc cagcgccccg gcgagcggct ggacctggct atgctggctg cgattcgccg
     4261 cgtttacggg ctacttgcca atacggtgcg gtatctgcag tgcggcgggt cgtggcggga
     4321 ggactgggga cagctttcgg ggacggccgt gccgccccag ggtgccgagc cccagagcaa
     4381 cgcgggccca cgaccccata tcggggacac gttatttacc ctgtttcggg cccccgagtt
     4441 gctggccccc aacggcgacc tgtataacgt gtttgcctgg gccttggacg tcttggccaa
     4501 acgcctccgt tccatgcacg tctttatcct ggattacgac caatcgcccg ccggctgccg
     4561 ggacgccctg ctgcaactta cctccgggat ggtccagacc cacgtcacca cccccggctc
     4621 cataccgacg atatgcgacc tggcgcgcac gtttgcccgg gagatggggg aggctaactg
     4681 atcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     4741 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     4801 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     4861 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     4921 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
     4981 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     5041 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     5101 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     5161 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     5221 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     5281 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     5341 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     5401 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     5461 catcttgttc aatggccgat cccatggtgg cggcgtcgac aataacttcg tataaagtat
     5521 cctatacgaa gttataggtc gaaaggcccg gagatgagga agaggagaac agcgcggcag
     5581 acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga ggaccttcgg gcgcccgccc
     5641 cgcccctgag cccgcccctg agcccgcccc cggacccacc ccttcccagc ctctgagccc
     5701 agaaagcgaa ggagcaaagc tgctattggc cgctgcccca aaggcctacc cgcttccatt
     5761 gctcagcggt gctgtccatc tgcacgagac tagtgagacg tgctacttcc atttgtcacg
     5821 tcctgcacga cgcgagctgc ggggcggggg ggaacttcct gactagggga ggagtagaag
     5881 gtggcgcgaa ggggccacca aagaacggag ccggttggcg ctaccggtgg atgtggaatg
     5941 tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg ctaaagcgca
     6001 tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaattgcg gccagcttgc
     6061 ggccatccaa ctatgaaaca ttatcataaa gcaatagtaa agagccttca gtaaagagca
     6121 ggcatttatc taatcccacc ccacccccac ccccgtagct ccaatccttc cattcaaaat
     6181 gtaggtactc tgttctcacc cttcttaaca aagtatgaca ggaaaaactt ccattttagt
     6241 ggacatcttt attgtttaat agatcatcaa tttctcgact attcctttgc cctcggacga
     6301 gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc ggtccagacg
     6361 gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt
     6421 gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac caagctctga
     6481 tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga tcctgcaagc
     6541 tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc
     6601 cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc gctccagtca
     6661 atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa atccgcgtgc
     6721 acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc gagagcctgc
     6781 gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac atggggatca
     6841 gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg cggtccgaat
     6901 gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat ggcctccgcg
     6961 accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt gacaccctgt
     7021 gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc aagcacttcc
     7081 ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt gtagaaacca
     7141 tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa gctgaaagca
     7201 cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa cttttcgatc
     7261 agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatggtggc ggcggctagc
     7321 gataccgtcg atccccactg gaaagaccgc gaagagtttg tcctcaaccg cgagctgtgg
     7381 aaaaaaaagg gacaggataa gtatgacatc atcaaggaaa ccctggacta ctgcgcccta
     7441 cagatctgca gcccggggga tccactagtt ctagcctcga gtagtccaat ttgttaaaga
     7501 caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat
     7561 agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     7621 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     7681 gaaaaataca taactgagaa tagagaagtt cagggaagcg gggcgcatca tatcaaatga
     7741 cgcgccgctg taaagtgtta cgggacatct actttgtgca tgacacaagc cagtgggtca
     7801 gaagtttaca tacactgaag ttcctatact ttctagagaa taggaacttc ttgagaaaga
     7861 attgggatcc cgatcaaggt caggaacaga tggaacagct agagaaccat cagatgtttc
     7921 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     7981 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     8041 tcactcggcg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
     8101 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     8161 gtgattgact acccgtcagc gggggtcttt cacccagagt ttggaactta ctgtcttctt
     8221 gggagggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     8281 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8341 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8401 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8461 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8521 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     8581 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     8641 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     8701 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     8761 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     8821 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     8881 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     8941 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9001 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9061 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9121 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9181 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9241 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9301 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     9361 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     9421 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     9481 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     9541 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     9601 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     9661 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     9721 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     9781 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     9841 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     9901 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     9961 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10021 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10081 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10141 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10201 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10261 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10321 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    10381 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
